![]() Title: The Process of Determining the Unknown Bacteria #9 Rachel Judecki July 5, 2011 2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to. Methyl Red (MR) and Voges-Proskauer (VP) broth is used as a part of the IMViC tests as the medium in which both the Methyl Red and Voges-Proskauer tests can be performed. In the attached image, I underlined each NP in section E. Linguistic questions. Hi Jada, Thanks for choosing me as your tutor! It is a simple broth that contains peptone, buffers, and dextrose or glucose. To perform these tests a loop full of the bacteria is added to the broth and incubated. Methyl red is a dye that is acid-sensitive. It turns yellow at a pH above 4.5 and when the pH is below 4.5 it turns red. The Methyl Red test involves adding the pH indicator methyl red to an inoculated tube of MR-VP broth. If the organism uses the mixed acid fermentation pathway and produces stable acidic end-products, the acids will overcome the buffers in the medium and produce an acidic environment in the medium. Microbiology Lab Reports BacteriaHOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to. Bacteriology Lab Floor PlanWhen methyl red is added, if acidic end products are present, the methyl red will stay red. The VP test detects organisms that utilize the butylene glycol pathway and produce acetoin. When the VP reagents are added to MR-VP broth that has been inoculated with an organism that uses the butylene glycol pathway, the acetoin end product is oxidized in the presence of potassium hydroxide (KOH) to diacetyl. Creatine is also present in the reagent as a catalyst. Diacetyl then reacts to produce a red color. Therefore, red is a positive result. If, after the reagents have been added, a copper color is present, the result is negative. Neither of these tests were performed during this experiment. The test Related Documents •. Clearing would appear around the disk containing the antibiotic. The diameter of this circle was then measured and compared with a table found on page 95 of the Microbiology Lab Manual to understand the antibiotics resistance, intermediate and sensitive levels, as they differ for each antibiotic. Bacteriology LabThe numbers on the table in the Microbiology Lab Manual represented whether the microbe was resistant, shown in the light blue color on the graph, intermediate, shown in purple, or sensitive to the antibiotic. Learned so far in the microbiology laboratory class for the identification of unknown bacteria. Bacteriology Lab TechniquesThe identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation and recording of data the unknown organism can be found. Instructions for the final laboratory report Your Full Name Cebu Institute of Technology - University [email protected] Abstract The abstract should have a maximum of 100 words and should briefly state the problem, method, and the summary of the lab report. Lab reports can be computerized or handwritten with 2 cm (1 in) left and right indention for the abstract. Introduction Paper to be used is 8.5” x 11” (letter or short bondpaper). There will be a 1-inch or 25.4 mm margin on all. Citation, Figure, Hypotenuse 642 Words| 3 Pages. THE DEPARTMENT OF PSYCHOLOGY GUIDE TO WRITING RESEARCH REPORTS The following set of guidelines provides psychology students at Essex with the basic information for structuring and formatting reports of research in psychology. Bacteriology Lab Manual![]() During your time here this will be an invaluable reference. You are encouraged to refer to this document each time you write a lab report. The writing of laboratory reports is an essential part of any practical module in Psychology. BacteriologyThis is because psychologists (and more. Arithmetic mean, Effect size, Lab Report 5309 Words| 10 Pages. UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease.
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